202 research outputs found
High-Resolution Optical Studies on C-Phycocyanin via Photochemical Hole Burning
We have shown that both the native C-phycocyanin and its corresponding free biline chromophore undergo reversible,
low-temperature photochemistry. We attribute this photochemistry to reversible proton-transfer processes and utilize the observed photoreaction for photochemical hole burning (PHB). Using narrow-band PHB experiments, we have been able to perform high-resolution optical studies and show that the protein-chromophore assembly forms a very rigid structure. The results lead to the conclusion that the light-induced proton transfer occurs most probably in the triplet state
Quasi-degenerate self-trapping in one-dimensional charge transfer exciton
The self-trapping by the nondiagonal particle-phonon interaction between two
quasi-degenerate energy levels of excitonic system, is studied. We propose this
is realized in charge transfer exciton, where the directions of the
polarization give the quasi-degeneracy. It is shown that this mechanism, unlike
the conventional diagonal one, allows a coexistence and resonance of the free
and self-trapped states even in one-dimensional systems and a quantitative
theory for the optical properties (light absorption and time-resolved
luminescence) of the resonating states is presented. This theory gives a
consistent resolution for the long-standing puzzles in quasi-one-dimensional
compound A-PMDA.Comment: accepted to Phys. Rev. Letter
Microscopic derivation of Frenkel excitons in second quantization
Starting from the microscopic hamiltonian describing free electrons in a
periodic lattice, we derive the hamiltonian appropriate to Frenkel excitons.
This is done through a grouping of terms different from the one leading to
Wannier excitons. This grouping makes appearing the atomic states as a relevant
basis to describe Frenkel excitons in the second quantization. Using them, we
derive the Frenkel exciton creation operators as well as the commutators which
rule these operators and which make the Frenkel excitons differing from
elementary bosons. The main goal of the present paper is to provide the
necessary grounds for future works on Frenkel exciton many-body effects, with
the composite nature of these particles treated exactly through a procedure
similar to the one we have recently developed for Wannier excitons.Comment: 16 pages, 4 figure
High-Resolution Optical Studies on C-Phycocyanin via Photochemical Hole Burning
We have shown that both the native C-phycocyanin and its corresponding free biline chromophore undergo reversible,
low-temperature photochemistry. We attribute this photochemistry to reversible proton-transfer processes and utilize the observed photoreaction for photochemical hole burning (PHB). Using narrow-band PHB experiments, we have been able to perform high-resolution optical studies and show that the protein-chromophore assembly forms a very rigid structure. The results lead to the conclusion that the light-induced proton transfer occurs most probably in the triplet state
Novel Interactions between Actin and the Proteasome Revealed by Complex Haploinsufficiency
Saccharomyces cerevisiae has been a powerful model for uncovering the landscape of binary gene interactions through whole-genome screening. Complex heterozygous interactions are potentially important to human genetic disease as loss-of-function alleles are common in human genomes. We have been using complex haploinsufficiency (CHI) screening with the actin gene to identify genes related to actin function and as a model to determine the prevalence of CHI interactions in eukaryotic genomes. Previous CHI screening between actin and null alleles for non-essential genes uncovered ∼240 deleterious CHI interactions. In this report, we have extended CHI screening to null alleles for essential genes by mating a query strain to sporulations of heterozygous knock-out strains. Using an act1Δ query, knock-outs of 60 essential genes were found to be CHI with actin. Enriched in this collection were functional categories found in the previous screen against non-essential genes, including genes involved in cytoskeleton function and chaperone complexes that fold actin and tubulin. Novel to this screen was the identification of genes for components of the TFIID transcription complex and for the proteasome. We investigated a potential role for the proteasome in regulating the actin cytoskeleton and found that the proteasome physically associates with actin filaments in vitro and that some conditional mutations in proteasome genes have gross defects in actin organization. Whole-genome screening with actin as a query has confirmed that CHI interactions are important phenotypic drivers. Furthermore, CHI screening is another genetic tool to uncover novel functional connections. Here we report a previously unappreciated role for the proteasome in affecting actin organization and function
Non-Hermitian Localization and Population Biology
The time evolution of spatial fluctuations in inhomogeneous d-dimensional
biological systems is analyzed. A single species continuous growth model, in
which the population disperses via diffusion and convection is considered.
Time-independent environmental heterogeneities, such as a random distribution
of nutrients or sunlight are modeled by quenched disorder in the growth rate.
Linearization of this model of population dynamics shows that the fastest
growing localized state dominates in a time proportional to a power of the
logarithm of the system size. Using an analogy with a Schrodinger equation
subject to a constant imaginary vector potential, we propose a delocalization
transition for the steady state of the nonlinear problem at a critical
convection threshold separating localized and extended states. In the limit of
high convection velocity, the linearized growth problem in dimensions
exhibits singular scaling behavior described by a (d-1)-dimensional
generalization of the noisy Burgers' equation, with universal singularities in
the density of states associated with disorder averaged eigenvalues near the
band edge in the complex plane. The Burgers mapping leads to unusual transverse
spreading of convecting delocalized populations.Comment: 22 pages, 11 figure
Nonequilibrium spectral diffusion due to laser heating in stimulated photon echo spectroscopy of low temperature glasses
A quantitative theory is developed, which accounts for heating artifacts in
three-pulse photon echo (3PE) experiments. The heat diffusion equation is
solved and the average value of the temperature in the focal volume of the
laser is determined as a function of the 3PE waiting time. This temperature is
used in the framework of nonequilibrium spectral diffusion theory to calculate
the effective homogeneous linewidth of an ensemble of probe molecules embedded
in an amorphous host. The theory fits recently observed plateaus and bumps
without introducing a gap in the distribution function of flip rates of the
two-level systems or any other major modification of the standard tunneling
model.Comment: 10 pages, Revtex, 6 eps-figures, accepted for publication in Phys.
Rev.
A cytomorphological and immunohistochemical profile of aggressive B-cell lymphoma: high clinical impact of a cumulative immunohistochemical outcome predictor score
We analyzed morphological and immunohistochemical features in 174 aggressive B-cell lymphomas of nodal and extranodal origin. Morphological features included presence or absence of a follicular component and cytologic criteria according to the Kiel classification, whereas immunohistochemical studies included expression of CD10, BCL-2, BCL-6, IRF4/MUM1, HLA-DR, p53, Ki-67 and the assessment of plasmacytoid differentiation. Patients were treated with a CHOP-like regimen. While the presence or absence of either CD10, BCL-6 and IRF4/MUM1 reactivity or plasmacytoid differentiation did not identify particular cytomorphologic or site-specific subtypes, we found that expression of CD10 and BCL-6, and a low reactivity for IRF4/MUM1 were favourable prognostic indicators. In contrast, BCL-2 expression and presence of a monotypic cytoplasmic immunoglobulin expression was associated with an unfavourable prognosis in univariate analyses. Meta-analysis of these data resulted in the development of a cumulative immunohistochemical outcome predictor score (CIOPS) enabling the recognition of four distinct prognostic groups. Multivariate analysis proved this score to be independent of the international prognostic index. Such a cumulative immunohistochemical scoring approach might provide a valuable alternative in the recognition of defined risk types of aggressive B-cell lymphomas
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